Abstract
For the purpose of hydrogen production with the wastewater from anaerobic hydrogen fermentation process, the enrichment of halotolerant photosynthetic hydrogen producing bacteria (PHPB) from tideland sediment sample with three lower volatile fatty acids (VFAs) as the substrate was carried out. The enriched halotolerant PHPB culture was obtained by serial subculture method with malic acid as a substrate at first. The enriched culture produced hydrogen of 1mol-H2/mol-acetic acid, 1.3 mol-H2/mol-lactic acid and 4.0 mol-H2/mol-butyric acid under anaerobic condition at 30 ℃, pH 7.0 and 6,000 lx of light irradiation. In addition, the all VFAs were consumed completely during hydrogen production period, thus it was considered that the enriched PHPB culture was useful for not only hydrogen production but also wastewater treatment from anaerobic hydrogen fermentation process.
