2025 Volume 61 Issue 2 Pages 19-30
We developed a method for the mass cultivation of the cyanobacterium Anabaena macrospora （syn. Dolichospermum hangangense） and for the mass production and collection of dormant cells, successfully germinating these dormant cells after a specified dormancy period. In this study, by using this method to evaluate the germination of dormant cells, we assessed how water temperature and oxygen conditions during dormancy affect the survival and germination of these cells.
The results indicated that dormant cells maintained a high survival rate at low temperatures, whereas their survival rate diminished at high temperatures. Furthermore, dormant cells demonstrated a high survival rate under anaerobic conditions but perished in aerobic conditions. It was also discovered that dormant cells can germinate after more than three months of dormancy. They typically germinate synchronously when environmental temperatures shift from low to high, but they rarely germinate when temperatures change from high to low. Dormant cells that did not germinate at high temperatures during dormancy may germinate when exposed to low temperatures. This indicates that dormant cells can enter a state of secondary dormancy.
