Japanese Journal of Water Treatment Biology
Online ISSN : 1881-0438
Print ISSN : 0910-6758
ISSN-L : 0910-6758
A Simple Method for Counting Escherichia coli Sensitive and Resistant to Antibiotics in a Bacterial Community
ZEN'ICHIRO KAWABATAMAN-GI MINKAZUAKI MATSUINOBUYOSHI ISHII
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1996 Volume 32 Issue 1 Pages 61-66

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Abstract

In order to count Escherichia coli both sensitive and resistant to ampicillin in a paddy field community, the quantity of Colilert, the time for incubation, and concentration of ampicillin were studied. Colilert was dissolved with sterilized distilled water and dispensed into a 1.5ml centrifuging tube and the MPN method under 30- to 70-hours incubation was adopted for enumeration of E. coli. Commercial Colilert could be reused 20 times. Colilert containing 0.5mg/l of ampicillin was applicable for counting E. coli resistant to ampicillin.

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