Abstract
The growth and development of human conjunctival epithelial cells on threedimensional collagen gels were influenced by the type of fibroblasts dispersed in the collagen gels and by the exposure of confluent epithelial cells to the air-liquid interface. By plating primary human conjunctival epithelial cells on Swiss 3T3-dispersed collagen gels and their subsequent exposure to air-liquid interface upon confluent, a conjunctival equivalent with characteristics of a real conjunctival epithelium was developed.