抄録
Various sugar derivatives having carboxylic acid group, e. g., D-glucuronic acid, D-galacturonic acid, D-mannuronic acid, sialic acids,3-deoxy-D-manno-2-ocutulosonic acid (KDO), Lipopolysaccharides (LPSs), lactobionic acid, and Nacetylmuraminic acid were immobilized on aminomethylated polystyrene dishes, and murine macrophage cell line TME9 cells or murine peritoneal exudate cells were cultured on the immobilized dishes. In the supernatants of those cells, high productions of tumor necrosis factor (TNF), interleukin- I (IL-I), IL-6, granulocyte-macrophage-colony stimulating factor (GM-CSF), and granulocytestimulating factor (G-CSF) were detected. TME9 cells released TNF, IL-6, and GMCSF at the early stage of the culture, and IL- I at the second stage.
