抄録
In this article, pathological studies of glomerulopathy using cultured glomerular visceral epithelial cells (GEC) are described. The mechanism of puromycin aminonucleoside (PAN)-inducedn ephrosis, which is similar to minimalc hange disease in humans, has not yet been well defined. We investigated roles of active oxygen species in GEC injury in vitro induced by PAN. Concomitant addition of either hydrogen peroxide scavenger or iron chelating agent to the culture medium with PAN caused a striking reduction of cellular injury. The release of hydrogen peroxide into the medium by cultured GEC exposed to PAN was shown to increase dose-dependently. These results strongly suggested that active oxygen species might play an important role in PANinduced GEC injury in vitro as well as in vivo. Moreover, we also found that injection of antibodies raised against membrane fractions purified from cultured GEC to rats induced rapid and severe proteinuria associated with GEC damage. Thus, these experimental models hopefully will lead to a greater understanding of the mechanism by which proteinuria is induced and developed.
