Abstract
Membrane associated SI factor, LIF(Leukemia inhibitory factor)and bFGF synergistically stimulate proliferation of murine primordial germ cells in primary culture. Moreover, pluripotential embryonic germ cell line can be derived from primordial germ cells after prolonged culture. Although growth of primordial germ cells in primary culture is affected by genetical background, we have successfully got EG cell lines from all inbered mice that we tested. STO feeder cells seem to provide unknown factor that may be important for EG cells to remain in undifferentiation.
