組織培養研究
Online ISSN : 1881-3704
Print ISSN : 0912-3636
ISSN-L : 0912-3636
IMPROVED TRANSFECTION EFFICIENCY OF 3Y1 AND ITS DERIVATIVE CELLS
Yoshiko HATAKEYAMANaohiro TSUYAMATakayo SASAKIToshinori IDE
著者情報
キーワード: 3Y1, transfection, electroporation
ジャーナル フリー

1995 年 14 巻 2 号 p. 141-145

詳細
抄録
Ttransfection efficiency for permanent transfectants was improved in 3Y1 and its derivative cell lines. Transfection efficiency of various cell cycle ts mutant clones and an adenovirus transformed clone showed transfection efficiency at 10-4-10-5 and 10-5-10-6, respectively, by calcium phosphate precipitation method including various modifications. The efficiency was improved up to 10-3 by electroporation in all these clones as well as parental 3Y1.
著者関連情報
© The Japanese Tissue Culture Association
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