抄録
We have developed a new skin model which was composed of two types of collagen sponge and two types of human cells. This skin model has two layers system, dermis and epidermis, like a real skin. In immunohistochemical study, differentiation markers of epidermis, high-molecularweight cytokeratin and involucrin, were detected in the skin model.
Furthermore, we performed an alternative study of animal testing using the skin model. Ten test chemicals were evaluated their irritancy with M I I assay which evaluates cell viability by measurement of its mitochondria's dehydrogenase activity. In the result, the skin model could distinguish non- and severe irritant.
