Abstract
We studied expression of cDNAs for human erythropoietin (EPO) and granulocyte-macrophage colony-stimulating factor (GM-CSF) in human lymphoblastoid Namalwa cells. The following results indicate that Namalwa cells have desirable characteristics as a host cell line to produce recombinant human glycoproteins: i)High-level expression such as 1-2μg/106 cells/day for EPO and 10-20μg/106 cells/day for GM-CSF was achieved. ii) Exogenous DNA was amplified in Namalwa cells. iii) Almost all N-linked oligosaccharides of Namalwa-derived EPO were shared by human urinary EPO. iv) Highly expressive transformants were readily adapted to grow in serum-free medium.
