Abstract
Serum-free media developed for general use were examined for the capability of shortterm or long-term growth of various cell lines. The examined media were ASF 103, ASF 104, and GIT. Most established cell lines grew fairly or very well in these media, although slower growth was obvious in some cell lines at a lower cell inoculum. Primary cultured cells from Syrian hamster embryos also grew fairly well when EGF was added to these media. ASF 103 and GIT media supplemented with EGF supported the extended growth of Syrian hamster embryo cells, even though the control cells cultured in a serumcontaining medium showed in vitro senescence. These media had little effect on the production of IgG in hybridoma cell lines and of CA 19 - 9 in a stomach cancer cell line. Growth capacity of HeLa cell line in ASF 103 and GIT media having been kept at 4°Cor -20°C for as long as 9 months proved to be fairly good.
