Highly Sensitive Analysis of Metallothionein in Human serum by HPLC with N-(9-acridinyl) maleimide fluorometry

Abstract

The quantitative analysis of sulfhydryl group of Metallothionein was developed in the human serum for the biomarker of the stresses toward the environments. The serum was treated by diluted hydrochloric acid and the reducing agent under the precise conditions, and analyzed by HPLC with N-(9-acridinyl) maleimide fluorometry. Sulfhydryl group was detected in pico (10-12) mole level easily and sensitively.