Stability of Serum Lactate Dehydrogenase Activity and Isozyme Pattern in Cattle Based on Storage Time and Temperature

Abstract

This study aimed to assess the stability of serum lactate dehydrogenase (LDH) activity and isozyme pattern based on storage time and temperature in cattle. Serum from Holstein and Japanese black cattle was stored at a variety of temperatures (4, −20 and −80 ℃). We also examined the serum of swine (Yorkshire) as a control. In Holstein and Japanese black cattle, there were no significant differences in serum LDH activity and isozyme pattern for 12 months at −20 and −80 ℃. On the other hand, LDH activity of stored serum at −20 ℃ in swine decreased significantly (P＜0.05) after 1 month. The M subunit ratio of LDH in swine serum was high compared with bovine serum. It was suggested that LDH activity and isozyme pattern using the bovine serum stored at −20 ℃ was stabilized for 12 months.