Abstract
1. When Hayem's solution containing 0-1.0% HgCl2 was used in microscopical examination the blood, clumps of erythrocytes appeared more of less in all kinds of animals. They were very large in number in the blood of goats, not so large in that of sheep and swine, and very small that of horses, cattle, and dogs.
2. It was thought that such clump had been caused in the diluted blood of horses and cattle mainly by some factors existing in the blood plasma and in that of sheep, goats, swine, and dogs by some factors in the erythrocytes.
3. There were the following relations between the concentration of HgC12 in Hayem's solution and the species of animals in which hemolysis occurred when such solution had been used as diluent: under 0.25% in cattle, under 0.05% horses and dogs, and under 0.01% in goats, sheep, and swine.
4. Taking the clumping of erythrocytes and hemolysis into consideration, the optimum concentrations of HgC12 in Hayem's solution are as fallows; 0.25-1.0% for horses and dogs, 0.5% for cattle, and 0.05% for sheep, goats, and swine.
5. No clumping occurred in physiologic saline solution at all, but hemolysis appeared in the blood diluted with this solution in all domestic animals examined in a short time. Therefore, physiologic saline solution cannot be a suitable diluent for erythrocytes of domestic animals.
6. When blood was diluted with Gowers' solution, no clumping nor hemolysis occurred for 24 hours. So, this solution can be recommended as a satisfactory diluent.
7. Toisson's solution caused no clumping but sometimes hemolysis especially in swine.
