Abstract
The serum iodine test, a simplifyed clinical test as a substitute for the Gross test, has been applied to dairy cows since 1961. The following findings were obtained from the practice of this test.
1. The amount of. a single drop from a given pipette varied according to the condition in which the pipette was held. A rather constant amount of a drop could be obtained when a glass capillary of the same caliber as the syringe of 1/3 gauge was used carefully without shaking.
2. A new pipette must be used for a new serum sample. If a pipette is employed for two or more samples continually, no accurate test can be performed because of mixing of samples.
3. No homolyzed samples should be used, since they may give more intensified tests.
4. The reagent for the test could be used even over a year after preparation when it was stored in a container tightly sealed with a polyethylerfe stopper, protected from exposure to light and contact with metal.
5. Serum samples must be tested within 4 days after collected in summer, although it is not always necessary to keep them in a refrigerator.
