Propagation of Japanese Encephalitis Virus in Swine Testicle Cultured Cells

Abstract

The virulent Sagara strain and the attenuated Sstrain of Japanese encephalitis virus were successfully propagated in swine testicle cultured cells.
A severer cytophathogenic effect was induced by the virulent strain than by the attenuated one when incubation was done at 37°C. The virulent strain gave a maximum virus yield (10⁸TCID₅₀/ml) in the find phase of cultures incubated at 37°C.
The attenuated strain gave a maximum virus yield.(10^6.0TCID₅₀/ml) in the cell phase of cultures incubated at 30°C. Swine testicle cells seemed to be quite suitable for the propagation of Japanese encephalitis virus.