Abstract
A simple and sensitive method for determination of residual sulfadimidine (SDD) in swine muscles and urine by high performance liquid chromatography (HPLC) was developed. Muscles were deproteinized with trichloroacetic acid. SDD in urine was extracted with chloroform. The deproteinized supernatant from the muscles and urine extract were reacted with o-phthaladehyde, and then injected into the HPLC column (Inertsil ODS-2). SDD was separated with a solution containing 1% phosphoric acid, methanol, and acetonitrile (62: 17: 21) as a mobile phase and detected with a spectrofluorometric detector (EX, 285 nm; EM, 445 nm). The recoveries of SDD from swine muscles and urine fortified with 400 ng were 78.1% and 89.6%, respectively. The limit of detection was 0.05 ng/mland it was adequate for residual analysis. In the experimental administration of SDD to swine, muscle SDD was not detected by this method after urine SDD completely disappeared. So it was indicated that the detection of SDD in urine is a useful indicator of SDD residual in the swine muscle.
