Immunohistochemical Evaluation of Amyloidosis in Animals

Abstract

In amyloidosis of various animal species, the changes in affinity for congo red after the pottasium permanganate treatment were compared with the amyloid protein determined by the immunohistochemical technique. Many cases examined showed sensitivity regarding the potassium permanganate treatment losing affinity for congo red (cows: 21/27; cats: 1/6; pigs: 2/2;dogs: 1/1; rabbits: 1/1; mice: 0/1; birds: 6/8) and reacted positively to the anti-AA antibody. Nearly all of the other cases showing some resistance to the potassium permanganatetreatment and retaining affinity for congo red (cows: 6/27; cats: 5/6; mice: 1/1; birds: 2/8) also reacted positive for the anti-AA antibody (cows: 6/6; cats: 5/5; mice: 0/1; birds: 1/2). “Ham spleen” in rabbits and cats showed an AA-protein deposit in contrastto the AL-protein in human beings. From these facts, it seems to be difficult to determine amyloid protein in animals using the pottasium permanganate treatment and the pattern of amyloid deposit.