Evaluation of Canine Neutrophil Adhesion Using a Coverslip

Abstract

Neutrophil adhesion in healthy dogs was evaluated using a coverslip method. Adhesion was revealed as the adhesive index calculated from the neutrophil numbers on a coverslip and those in the blood sample. The adhesive index decreased significantly after ethanol was added to the blood sample. The results suggested that the evaluation of canine neutrophil adhesion was possible using this method. The adhesive index in 46 healthy dogs was 38.3±11.6 (mean±S.D.). As this coverslip method is simple and rapid with no special equipment needed, it may be useful in assessing the functions of canine neutrophils.