RT-PCRによるペスチウイルスの検出と制限酵素切断パターンによる識別

山口 修; 迫田 義博; 佐藤 満雄; 中村 成幸; 福所 秋雄

doi:10.12935/jvma1951.50.639

Journal of the Japan Veterinary Medical Association

Online ISSN : 2186-0211
Print ISSN : 0446-6454
ISSN-L : 0446-6454

Gene Detection and Discrimination of Pestivirus Strains by RT-PCR and Restriction Endonuclease Analysis

Osamu YAMAGUCHI, Yoshihiro SAKODA, Mitsuo SATO, Shigeyuki NAKAMURA, Akio FUKUSHO

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Keywords: bovine viral diarrhea virus, classical swine fever virus, pestivirus, restriction endonuclease analysis, RT-PCR

JOURNAL FREE ACCESS

1997 Volume 50 Issue 11 Pages 639-644

DOI https://doi.org/10.12935/jvma1951.50.639

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Abstract

All genes of classical swine fever viruses (CSFV) and bovine viral diarrhea viruses (BVDV) were detected by RT-PCR using primers located in the 5' non-coding region. Discrimination between CSFV and BVDV was performed easily by restriction endonuclease (>Bgl I andPstI) analysis of amplified PCR fragments.

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