Journal of the Japan Veterinary Medical Association
Online ISSN : 2186-0211
Print ISSN : 0446-6454
ISSN-L : 0446-6454
Molecular Epidemiology of Enterohemorrhagic Escherichia coli from Human Cases in Yamaguchi Prefecture
Masaaki TOMITAAtsushi KATAYAMAAkira IWASAKI
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1999 Volume 52 Issue 6 Pages 383-387

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Abstract

An epidemiological analysis of enterohemorrhagic E. coli from human cases in Yamaguchi Prefecture in 1996 was done using drug susceptibility patterns, plasmid profiles and pulsed-field gel electrophoresis (PFGE). Of 27 isolates, one was resistant to ABPC, SM and TC, while the other two were to TC and CP, respectively. By combination of PFGE and plasmid profile analysis, the isolates could be divided into 16 patterns, revealing that PFGE using several restriction endonucleases was useful for discrimination of different batcterial strains. Fifteen PFGE patterns were further divided into three groups, and 24 of 27 isolates were of the PFGE group I, suggesting that a single bacterial clone widely spread with variation in PFGE patterns.

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