Abstract
Bacterial and parasitic pathogens were negative in fecal samples taken from three adult diarrheal cows on a dairy farm in Akita Prefecture in January 2001, Electron microcopy revealed only rotavirus particles in the samples. RNA-polyacrylamide gel electrophoresis detected group-A rotavirus (GAR) RNA segments. GAR was isolated from two samples with MA104 cells, the VP7 serotype of which was G6 and the VP4 genotype of which was P [1] Significant GAR antibody response was observed in two affected and one non-affected cow. Results indicate that GAR infection caused diarrhea in the adult cows in this study.