Urinary Metabolic Profiles of Growth-retarded Japanese Black Cattle by Gas Chromatography/Mass Spectrometry Analysis

Abstract

To detect metabolic abnormalities, we performed gas chromatography/mass spectrometry (GC/MS) on growth-retarded Japanese Black cattle. Many organic acids, amino acids, sugars, nucleic-acid bases, and glycine conjugates were detected in urine from 18 normal, cattle. Increased excretion of orotic acid was observed in one growth-retarded calf. In 3 calves with renal tubular dysplasia, concentrations of hippurate, suberate, and phenols tended to be higher and concentrations of propionylglycine lower than in normal calves. In 14 other growth-retarded calves, concentration of hippurate was higher and concentration of propionylglycine tended to be lower than in normal calves. Concentrations of organic acids (4-hydroxyphenylacetate, methylcitrate, cis-aconite, and glycolate) and amino acids tended to be lower.