Abstract
Primary symptoms of sneezing and nasal bleeding, and histopathological findings of an intranasal biopsy specimen suggested aspergillosis in an outdoor-raising Golden Retriever, although neither culture nor PCR of biopsy samples detected any fungi. Nonetheless, local infusion of clotrimazole greatly improved purulent nasal discharge. We therefore diagnosed this case aspergillosis. This case suggests the impossibility of detecting nasal aspergillosis on the basis of a fungal culture or PCR of multiple biopsy samples.