Abstract
Previously, the present author""" examined the value of the cell count inmilk and BCP, BTB and CL reactions in the diagnosis of mastitis and reportedthat great care should be taken in the evaluation. In the present study, toobserve the qualitative changes of cells in the milk obtained from cows andgoats with baci11ar3r mastitis (A 1 -32), whose colostrum was stagnated (B 1 -11), on which milking had been interrupted (B12 -51) and which were healthy, microscopical examinations were made on the pathological changes of peroxidasein cytoplasm by copper peroxidase reaction after fixing milk with 4X.ORTHSsolution. It was found that, in mastitis, a peculiar change was observable incytoplasm.1. During bacillary mastitis, leucocytes, especially neutrophile ones, in-creased in nurnber and the formation of conglomerulations of various sizes wascharacteristic in both acute and chronic inflammations. Peroxidase in the cyto-plasm at the center of the conglomerulation had disappeared (Fig. 2, 3, 5) andthat at the periplreral part showed disturbed arrangement and peripheral dis-tribution (Fig. 4, 6).2. No conglomerulation of cells was observed in the milk from healthyanimals (Fig. 1).3. In some cases of stagnation of colostrum, or interrupted milking, epi-thelial cells of the gland and conglomerulation of leucocytes (Fig. 7, 8) werefound, though the above stated disappearance of peroxidase was not observed.These conglomerulations were resolved as time elapsed (usually after 5 days)and no new infiltration of leucocytes took place. 4. The more marked the disappearance of peroxidase of conglomerulations, the more intense was the catalase reaction : an antagonism was observed be-tween them.
