CULTURAL STUDIES ON LFPTOSPIRAE : I. ON THE GROWTH OF LEPTOSPIRAE IN LIQUID MEDIUM INACTIVATED AT 100°C AND 121°C

Abstract

Attempts were made to inactivate the liquid medium of leptospira at high temperatures, such as 100°C and 121°C, after the addition of rabbit serum. The growth of leptospira was generally more increased in the media which were inactivated at 100°C or 121°C than in those which were inactivated at 56°C or not inactivated. The same phenomenon was observed in the medium to which was added horse, cattle and sheep serum, respectively. In the medium inactivated at 121°C, however, occasional poor growth or inhibition of leptospira was demonstrated.