Abstract
The observations obtained from the experiments on the antigenicity and allergenicityof Setariatoxin are summerized as follows :1) The M.L.D. (minimum lethal dose) of crude Setariatoxin was found to be 30mg/kg, when given to the guinea pig through intravenous injection (Table 1).2) The crude Setariatoxin (C.S.) was not hemolytic for the blood of rabbits andguunea pugs.3) The Setariatoxin can be divided into fractions as follows : Ether supernatant liquidfraction, ethvl alcohol sur>ernatant lictuid fraction 3, and ethyl alcohol sediment fraction(T.bl. 2).4) The nature of each fraction was as follows : Ether supernatant liquid fraction isequal to the lipoid fraction (11) ; ethyl alcohol supernatant liquid fraction is equal to thepolysaccharide fraction (Ps), and ethyl alcohol sediment fraction is equal to the proteinfraction (Pr) (Table 3).5) The Shwartzman rhenomenon of each fraction (Cs, Ps, Pr, Li) was as follows :Li fraction was negative, but others (Cs, Ps, Pr) were positive (Table 4).6) The Prausnitz-Ktstner reaction with each fractiorr (Cs, Ps, Pr) and the rabbitimmune serum (against crude Setariatoxin) was found to be positive (Table 5).7) Crude Setariatoxin and its Ps fraction produced contraction of extirpated intestine ofnormal guinea pig by Schultz-Date method (Fig. l). The contraction was relaxed by theadministration of an antihistamic (Fig. 2).8) Crude Setariation, Ps and Pr fraction produced contraction of the extirpated intestineof the guinea pig sensitized with 1002 of crude Setariatoxin. The contraction with Prfraction seemed to be an allergic reaction (Figs. 3, 4). The contraction of the sensitizedintestirre by Ps fraction was inhibited by atropine sulfate.9) No contraction was obtairted by the administration of crude Setariatoxin and itsfractions as well as histamine on the extirpated i.ntestine of normal rat (Figs. 6, 7).
