Abstract
Since JOHNSON ([927) reported on the imnmunity of chickens infected witlx coccidium, rnan-)z -workers have stud-iecl this sulcject, but 11=O prodv, uctioin of ancibody in tl?te serun? couldbe clarified as yet. In tlaeir previov, us investigation (1955), the authors demonstrated thatthe rrterozoite was an agglutir?ogen showing excellent agglu-tinability. In this study, theyexamined, making use of the antigenieity of the ooc, zst>whetlaer annLibody vxas producedin t1?e serum of rabbits immtunized with the oocyst or not.To prepare antigens, oocysts NVCIC collected from the ceeal contents eonta, icing nurrnerousoocysts. For rurification* thev were vx"ashecl man>times with clisti1.lecl water OY centri-fv, tgation and treated finally with ether-a!cohyl.Imvr.unized arttigens to rabbits were prepared by Bachmans metlaod. III tlais method, pqxxzciered oocysts c, were stuspended ixt O.85% sa, lirue solvation. Tlae resulting suspension wasboiied Yor 4 hours 111 1 [)Cl CUI1t. SUSpCIlS1011 Of d1SL11lCCL zNdtCT. AS 2 ICSU.[t, It XV2S dlVldCdjxato sec[iment anvc[ supernatant, 1, coLh of wlaich XX"CTC irtjectecl to rabbits.Feat antmgens XVC1C) prepared ill sucll Q 1I1&111lC)I 3-S SI10XXIll 111 Cllart 1. In OdditiO11, the?cowxdered oocysts were subjected to extraccion with Cocas sulutiorn.For precipitation reaction, clae ring tesim, place method (Oacltterlora-y rnetI*od), and aIQOdifiC1tl0Il Of Ouduns II1Cthoc1 were used.Tlue results obtauned 3TC SUiITl1I1lFiZCd 2S f0NI0XVS.1. All the 2I1t19C11S D1CDlICCl frorn DO?ClC1Cd 00CVStS arrct bOi=IClCI seCli.ntents 01 suoer-DtrJL/t.aLat?.tS had ability to produce precipitirx in the serum of rabbits. The powclered oocystsj)TOd11CCCl pFCC1p1tiIl 21g1iIlSt both pot"y.saecluarrCIe and p1l0tC7.11. ThC bO1lCC1 SCC1i1I1CThtS XVCTCchiefly agamnsc protemn llld the bO1l.CC1 SLlpCUIllt3I1tS Og2L].l1St polysacc11arv.Cle.2. All th! test lIlt15C11S) IIICII-1Cll11& Cucas S0lllti0ll CXtI1Utj 3ClUS0llS ()h{tl1Ct) 9Y1C3. PONY-saccllarrde 3P -d PI0tClIl fl<ICLIOIIS>S
