Abstract
Growth of equine infectious anemia virus in equine leucocyte culture is influencedconsuderably by individuality of horse from which the leucocytes were derive. It alsoinfluenced by bovine serum which is contained in tissue culture medium No. 199.The relationship between the percentages of the serurn protein fractions o btainedby paper electrophoresis and the results of leucocyte culture was investigated to elucidatethe nature of the most adequate serum for equine leucocyte culture.As a result, the percentages of the albumin and the beta-globulin fraction were low, and that of the alpha-globulin fraction was high in such sera as considered adequatefor equine leucocyte culture.To obtain a serum sample which has the nature described above, the serum sampleshould be collected aseptically. it should not be filtered through a bacterial filter.If a serum sample is not filtered but heated at a proper temperature for a propertime (50 -60C, 30 -40 minute), it will give good results irc leucocyte culture.
