Abstract
Under controlled environnnent (Fig. 1), colostrum-deprived piglets were raised in annsolator (Fig. 2). They had beent tal<en out of the uterus aseptically by hystereetomy 111an operating hood. IVIicrobioIogicaI checking was carried out on the circumstancesuntcler which the isolator was installed, as well as on these isolated piglets. The resultswere as follows.1. A controlled environrnent was maintained at 25C of atmospheric temperatureand 60% of humidity, using a Cambridge absolute air filter, model 43A, xxzhere micro-biological checking was perforrnecl on the wall, floor, and falling organisrns. NoOrganisms were detected in suclt .1111 environment as stc:rilized with formalin-gas. NOoacteria were recovered either from the wall or the floor of the isolators where specificpathogen free (SPF) piglets had been raised for 21 days (Table 4).2. Such organisms as Bacillus, Micrococcus, and nc>rx-pathogenic Eseherichia wereisolated frorrz each fecal samples collected from three-day-old SPF piglets fed pasteurizedrnilk. This Escherichia strain possessed an unknown serotype, and the source of it wasnot known. Ixx other words, the serotype of this Escherichia was not idential with anyone of those isolated from the motlaer sow and the feces of workers handling the piglets(T.1, 1. 6).3. The genera and numbers of organisms recovered from the contents of tlae cecumOf 21-day-old SPF piglets were as follows : 10" (per grarrn) of Escherichia, 10= - IO? ofMicrococcus, and [0" - 10- of Bacteroides. No orc>anisms of Enterobacteriaceae other.bthan Escherichia, Lactobacillus, Bificlobacterium, Streptococcus fecalis, Staphyloeoccus, Corynebacterium, Clostridium, and Veillonella, nor fungi were recovered frorrn thesepiglets (Table 7).4. Antibodies of Japanese encephalitis, entero virus, hemoagglutinatiung virus ofJapan (HVJ), swine para influenza, hog cholera, transmissible gastroenteritis (TGE), vere not observed in such SPF swine. No viruses were detected from these animals bythe tissue culture method using swine kid
