Abstract
mesenteric Nymph nodes found at the abattoirof acidfast organisms were isolated. Theirexamined for identification. The results ob-From the tuberculous lesions of swinein Tokachi-Shimizu, Hokkaido, 70 strainsbiochemical and serological features weretained are summarized as follows.I. The isolates were examinedfor biological and biochemical characteristics byusing 12 kinds of routine tests, which are generally employed for differentiating Runyonsgroups I, II, III, and IV. In these tests and also in B6NICKES amidase pattern, theyshowed very similar characteristics and were supposed to be ?Mycobacterium avium or M.intracellulave of Runyons group III.2. By the agglutination reaction, 63 strains were identified as type "Davis", 3 strainsas type "Illa", and one strain each as type "IV" and "new type". The remaining 2strains were considered to be "unclassified".3. The agglutination test was a useful method for the differentiation of M. aviumfrom M. intracellulare.4. Complement-fixation antigens of the serotypes of M. avium and some types ofM. intracellulare were prepared from the acetone-soluble fraction of the hot methanolextract by SCI[AEFERS method. They showed good antigenicity and stability. The corn-plement-fixation test was also proved to be type-specific, as well as the agglutination test, and a useful tool for the identification of Runyons group III mycobacteria.
