Abstract
The Obihiro strain of feline panleukopenia (FPL) virus agglutinated swine erythrocytes whichi had been examined in advance for sensitivity to hemagglutination (HA) by the virus. All the reagents for the HA test were made of 0.01 M phosphate-buffered saline solution adjusted to a pH of 6.8. Specific HA reaction was recognized after a 2 hours' incubation period in a refrigerator at 4°C. It was inhibited by specific immune serum to the virus in the hemagglutination-inhibition (HI) test. There was a certain correlation between HI and neutralizing antibody titers by almost all the feline serum somples tested. Viral hemagglutinin was shown to be stable to ether and heat. In the virus fluids HA titers were not lost after sonic treatment of the virus, but were completely lost after ultraviolet irradiation. The results are in almost complete agreement with the physicochemical properties of the infectivity of the virus in cat kidney cell cultures. Treatment of the virus with potassium periodate solution had essentially no effect on the adsorption or the elution of the virus with swine erythrocytes. The virus, however, failed to agglutinate erythrocytes pretreated with a valid concentration of receptor-destroying enzyme. On the basis of these findings, the significance of the parvovirus-erythrocyte interaction was discussed.
