Abstract
Two simian cell lines, JINET and vero, were inoculated with infectiouscanine hepatitis virus at a variety of input multiplicity. The viral effects observed onthem were compared with those on primary dog kidney cells. Infected cell cultures andrecovered viruses were subjected to serial passages.In JINET cells, virus titer remained constant without showing an increase. None ofinclusion body and hemagglutinating and complement-fixing antigens were detected.Cytopathic effect occurred only when virus of high input multiplicity was inoculated.Immunofluorescent viral antigen was visible in a small number of cells. In vero cells, specifically fluorescent cells and infective viruses appeared at lower levels than in JINETcells, and no other changes associated with the virus infection were detected. Comparisonof surface morphology was made by scanning electron microscopy among the three kindsof cell cultures. It revealed that patterns for every degree of cytopathic changes corre-sponded to those described above.
