Abstract
Studies were made to clarify the specificities of anti-lymphocyte antibodies produced by allo-skin transplantation and pregnancy in bitches. Nine cytotoxic antibodies, designated anti DLA-J1, -J2, -J3, -J4, -J5, -J6, -J7, -J8 and -J9, were obtained from eight of 17 sibling beagles after allo-skin transplantation and from a pregnant bitch. A two-stage microlymphocytotoxicity technique was used for the detection of antibody. The antibody titer was expressed as the reciprocal of the final serum dilution causing 30% cytotoxicity for skin-donor lymphocytes and the sire's lymphocytes. It ranged from 4 to 512. From the results of the serogram and X2 values determined by the screening test with 40 dog lymphocytes, the antibodies obtained were examined. Because the X2 values were 6.2, 4.0 and 7.3 in anti DLA-J1-J2, -J1-J8, -J2-J8, and 4.8 in anti DLA-J3-J6, it was presumed that anti DLA-J1 might be practically identical with anti DLA-J2 and -J8, and anti DLA-J3 with anti DLA-J6. The specificities of the nine antibodies obtained were classified into six kinds; that is, anti DLA-J1-J2-J8, -J3-J6, -J4, -J5, -J7 and -J9.
