Abstract
Horses were immunized with a multi-component vaccine consisting of OEP, protease toxoid and elastase toxoid to investigate the therapeutic effect of immunization on corneal ulceration due to Pseudomonas aeruginosa infection. Two of three horses inoculated subcutaneously five times with the multi-component vaccine were used for the protection test, and two nonvaccinated horses as controls. A suspension containing 5.2×107 cells of Pseudomonas aeruginosa IFO 3455 per milliliter was used for challenge in the protection test. Six drops of it were deposited once on the traumatized cornea. Of the two vaccinated horses, one was protected from the bacterial infection. The other was affected with corneal opacity and mild corneal ulcer, which was localized and not aggravated in spite of n6 treatment. On the other hand, the two control horses suffered from severe ulcer and abscess spreading all over the cornea, respectively.
