Osmotic Fragility of Erythrocytes in Mice, Rats and Rabbits with Coil Planet Centrifuge Method

Abstract

The osmotic fragility of erythrocytes was measured in mice, rats and rabbits by the coil planet centrifuge (CPC) method. Blood samples were driven through the osmotic gradient saline solution from 30 to 150 mOsm. In the case of rabbits, the saline solution was prepared from 30 to 200 mOsm. Effects on storage condition of blood samples were studied at cold temperature (4°C) and room temperature (29-32°C). Storage at room temperature: There was no significant change in osmotic fragility of rat or rabbit erythrocytes during the first 6 hours, whereas hemolysic start point (HSP) values increased significantly after 24 hours. CPC values of mice increased with the lapse of storage time until after 24 hours. Cold storage: No significant change in osmotic resistance was seen in rats or rabbits during 24 hours. HSP values of mice increased appreciably after 24 hours. The CPC value of heparinized blood was higher than that of EDTA-2Na added blood in mice and Tats. There was no difference in osmotic resistance between heparinized blood and EDTA-2Na added blood in rabbits. No difference was observed in osmotic resistance between cardiac and peripheral venous blood in rats or rabbits.