Abstract
Isolation of the chemotactic components in the extract from adult Metastrongylus apri (M. apri) possessing chemotactic activity for eosinophils, neutrophils and macrophages of guinea pigs, was attempted. The components were assumed to be in a fraction separated by salting-out with 33 to 50% saturation of ammonium sulphate. The majority of the activity was contained in one of two protein peaks resolved by gel-filtration with Sephadex G-100. The active peak, neighbouring that of bovine γ-globulin, was divided into several peaks showing leukotactic activities with isoelectric focusing. Component a focused at pH 4.8±0.2 was specific for only macrophages, while c focused at pH 7.4±0.2 for both macrophages and eosinophils. Component b focused at pH 6.7±0.2 was active for neuotrophils, macrophages and eosinophils, and component d found at pH 5.2±0.2 was specific only for neutrophils. A chemotactic activity for eosinophils was found in a predominant peak focused at pH 5.8 to 7.8. In these fractions, the highest activity was shown by component e, of a peak at pH 7.2±0.2.
