Abstract
To identify and quantify bovine monocytes in peripheral blood mononuclear cells (MNCs), five procedures were used: neutral red-, latex- and yeast- ingestion methods, peroxidase staining and alpha naphthyl acetate esterase (ANAE) staining. The yeast ingestion method proved to be a simple and suitable procedure for discrimination between monocytes and lymphocytes. ANAE staining was also useful for identifying monocytes. Simultaneous application of phagocytosis of yeast and ANAE staining was the most reliable procedure for the identification of bovine monocytes.
