Abstract
Virological and mycoplasmological examinations of Iriomote cats (Prionailurus iriomotensis) were performed. Totally 11 cats which were clinically healthy were examined. Attempts to isolate cytopathic viruses, mycoplasmas and ureaplasmas from swab samples of respiratory, rectal and urogenital sites came to be negative. Although no viral antibodies against major feline viruses except feline calicivirus (FCV) were detected, 80% of the cats had the neutralizing antibody against FCV to some degree.