Abstract
The transformation of mitogen stimulated bovine lymphocytes was studied using the glucose consumption test. After four days of incubation of lymphocytes, the glucose concentration in the culture medium was determined. The differences of glucose concentration between control and mitogen stimulated cultures indicated the blastogenetic activity of the lymphocytes. Of four mitogens used, phytohaemagglutinin and concanavalin A responded well whereas responces with lipopolysaccharide and pokeweed mitogen were weak. This simple method was useful in evaluating the blastogenesis of bovine lymphocytes for routine use.
