Application of Colorimetric Assay for Detection of Tumor-associated Antigens on Bovine Leukemic Cells

Abstract

A rapid colorimetric assay was used to quantitate the cytotoxic activity of monoclonal antibody against tumor-associated antigen (TAA) of bovine leukemia cells. The viability of specific antibody-treated target cells was assessed by using a tetrazolium dye that is reduced to a purple formazan by living but not dead cells. The results of cytotoxicity determined by colorimetric assay were similar to that of trypan blue dye exclusion method. Using this colorimetric assay, we detected TAA in peripheral blood lymphocytes (PBL) from leukemic cattle as well as in PBL from most cattle with lymphocytosis but no evidence of tumor.