Preparation of Monoclonal Antibodies against Marek's Disease Virus and Herpesvirus of Turkeys

Abstract

Fifty-two monoclonal antibodies specific for Marek's disease virus (MDV) and herpesvirus of turkeys (HVT) were established. Twenty-two of these antibodies were specific for three different serotypes. Of the remaining antibodies, 22 cross-reacted with all combinations of the three serotypes (1·2·3, 1·2, 1·3 and 2·3). Using the serotype-specific antibodies, 10 presumable serotyped MDV and HVT and 7 field isolates could be serotyped by indirect immunofluorescence test. One of 7 field isolates was identified to a serotype 2 MDV which has been isolated for the first time in Japan.