Abstract
Selective medium for the isolation of urinary corynebacteria from samples contaminated with feces or urine of cows was prepared by incorporating 200 μg/ml fosfomycin and 2 μg/ml amphotericin B into standard blood agar. Using this medium, distribution of the organisms was examined from the vulva and vaginal vestibule of 210 apparently healthy cows. C. pilosum was isolated from the vulva and vaginal vestibule at the ratio of 16.2%, and C. renale was isolated from the same sites at 5.7% and 6.6%, respectively. Number of colonies detected from the vulva was greater than that from the vaginal vestibule. The findings suggest that bovine vulva may be a suitable habitat for C.pilosum and C. renale, and the results correlated with the fact that these organisms adhered best to the epithelial celld of the vulva in in-vitro experiments.
