Abstract
α1-acid glycoprotein (α1AGP) in bovine lymphocytes was detected by indirect immunofluorescence antibody test on approximately 90% of peripheral blood lymphocytes as well as lymphocytes in lymph nodes and spleens. α1AGP isolated from bovine lymphocytes revealed the consequent 5 spots in an acid portion (pI 3.5-4.5) with molecular weight (M. W.) 27, 000 to 39, 000 daltons on two dimensional gel electrophoresis. α1AGP obtained from the supernatant of lymphocytes cultured with bovine interleukin 2 (IL-2) showed the consequent 3 spots with pI 3.5-4.0 and M. W. 29, 000-36, 000 daltons. The α1AGP concentration in non-stimulated lymphocyte was found to be 21ng/106 cells by radioimmunoassay. The α1AGP levels in lymphocytes cultured with IL-2, concanavaline A, and pokeweed mitogen were increased from the level in non-stimulated cells to 78, 58, 69 ng/106 cells on day 4, respectively. Furthermore, the α1AGP level was as high as 90 ng/106 cells in lymphocytes cultured with IL-2 for 35 days. α1AGP was detected in culture supernatants of lymphocytes with IL-2 and not with above mentioned mitogens. From these results, bovine α1AGP was confirmed to be synthesized in lymphocytes, and much more actively produced in lymphocytes stimulated with IL-2.
