Abstract
This paper describes a new high-speed micro PIV technique for measuring both of red blood cells velocity and plasma velocity to investigate rheology of blood flow in a microcirculation. The measurement system consisted of epi-fluorescent microscope equipped with high-speed camera with image intensifier and two sets of color filters. The technique was applied to in vitro blood flow through micro round tube of 100μm diameter. Velocity distributions of fluorescent dyed RBC and plasma seeded with fluorescent particle were obtained separately. The obtained velocity distribution achieved high spatial resolution with 9.0μm×2.2μm and high temporal resolution at 6000Hz. The technique is useful for investigation of blood rheology in a microcirculation.