1988 Volume 14 Issue 3 Pages 323-329
Extraction and separation of biochemical substances such as cholesterin and bile acid from foot and dark oil and ester oil, which are intermediate products in fish oil refining processes, were studied by a supercritical CO2 extraction method. Fatty acid ester, methanol and cholesterin were separated from the ester oil, and bile acid was concentrated intensively by using alcohol as a reformer. From saponified foots, a cholesterin concentration of 98% was obtained by combining solvent extraction using benzene and supercritical CO2 extraction. Almost all the used benzene was recovered. The mechanism of supercritical CO2 extraction and the optimum conditions to combine these extraction prosesses are discussed.
