1958 Volume 32 Issue 8 Pages 543-561
The red type strains (red colonies producing strains on the Endo medium) were inhibited in their growth in a lower concentration of fuchsin as compared with the white type strains. This inhibitory activity was inactivated by Na2 SO3, 1-cysteine and thioglycolic acid. acid. Fuchsin prolonged the lag phase of both types and inhibited the growth in logarithmic and stationary phase. However, the earlier Fuchsin was used, the greater was the activity, which coincided well with the morphological variations, i.e. variations of form and colour of red colonies on the Endo medium, variations of bacterial body and apparition of red granules. It was well explainable from the differential resistance of both types to fuchsin that the above mentioned phenomena were characteristic of the red type. Against various stains, disinfectants and antibiotics, the red type showed a lower resistance than the white type, except against potassium tellurate.
Neutral red, eosine yellow, pyronine, acrydine yellow, acryflaviEne, picric acid, carmine, indigocarmine and mercurochrom stained in a low concentration the colonies of both types, whereas fuchsin, crystall violet, methyl violet, gentiana violet and potassium tellurate did not.
The latters stained them in the highest concentration where the growth was just possible. Regardless to the agents employed, bacterial body demonstrated morphological variations, as the concentration rose. The most marked variations, however, were found with the following agents: fuchsin, crystall violet, methyl violet, gentiana violet, malachite green, brillant green, methyl green, cresol, sulfathiazol, penicillin G and ilotycin. Safranine T, neutral red, eosine yellow and carmine stained the bacterial body of both types diffusely.
Safranine T and neutral red stained them reddish in a low concentration and reddishbrown in the highest concentration where the growth was just possible.
Fuchsin, crystall violet, gentiana violet, methyl green, acrydine yellow and acryflavine stained the bacterial body of botn types in the highest concentration, but not in a low concentration.
