A pathogenic E. coli strain, Horie strain (O-28a. 28c: B₁₈) isolated from an Ekiri patient was reported by the author et al in Vol.31, 1957 of this journal. Meanwhille, 3 cases with dysenterylike symptoms caused by this agent were experienced by us. These 3 strains demonstrated identical biological properties with the Horie strain and differed a little from the Katwijk. The Horie strain possessed serologically common antigens with 0-28 and 0-42 and on the other hand an antigen specific to the strain.

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The effects of PVL and cortisone on the immunizing power of rabies vaccine were examined using mice and rabbits. The results were as follows.
1) Group A which had received rabies vaccine together with PVL 3 times withstood 8 times as great a MLD (minimum lethal dosis) as that of group B given 6 times the rabies vaccine alone.
2) On the contrary, group C (3 times rabies vaccine with cortisone) demonstrated 1/1000 MLD of group A. Likewise, group D (3 times rabies vaccine and then 3 times cortisone) showed 1/52.
3) With rabbits also, a rapid formation of neutralizing antibodies was observed by the combined vaccination with PVL. i. e., the group of combined vaccination with PVL showed 4 times as great a neutralisation value in 10 days and 2 times in 21 days as that of the group with the rabies vaccine alone.
4) The group which had received 4 mg/kg cortisone of body weight together with the rabies vaccine in successive days showed a low neutralisation value, 1/10 of the group of combined vaccination with PVL and 1) 3 of the group with the rabies vaccine alone in 10 days and 1/6 of the former and 1/2 of the latter group respectively in 21 days.
5) Cortisone increased the susceptibility of mice to rabies virus and reduced the body weight of rabbits.

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The red type strains (red colonies producing strains on the Endo medium) were inhibited in their growth in a lower concentration of fuchsin as compared with the white type strains. This inhibitory activity was inactivated by Na₂ SO₃, 1-cysteine and thioglycolic acid. acid. Fuchsin prolonged the lag phase of both types and inhibited the growth in logarithmic and stationary phase. However, the earlier Fuchsin was used, the greater was the activity, which coincided well with the morphological variations, i.e. variations of form and colour of red colonies on the Endo medium, variations of bacterial body and apparition of red granules. It was well explainable from the differential resistance of both types to fuchsin that the above mentioned phenomena were characteristic of the red type. Against various stains, disinfectants and antibiotics, the red type showed a lower resistance than the white type, except against potassium tellurate.
Neutral red, eosine yellow, pyronine, acrydine yellow, acryflaviEne, picric acid, carmine, indigocarmine and mercurochrom stained in a low concentration the colonies of both types, whereas fuchsin, crystall violet, methyl violet, gentiana violet and potassium tellurate did not.
The latters stained them in the highest concentration where the growth was just possible. Regardless to the agents employed, bacterial body demonstrated morphological variations, as the concentration rose. The most marked variations, however, were found with the following agents: fuchsin, crystall violet, methyl violet, gentiana violet, malachite green, brillant green, methyl green, cresol, sulfathiazol, penicillin G and ilotycin. Safranine T, neutral red, eosine yellow and carmine stained the bacterial body of both types diffusely.
Safranine T and neutral red stained them reddish in a low concentration and reddishbrown in the highest concentration where the growth was just possible.
Fuchsin, crystall violet, gentiana violet, methyl green, acrydine yellow and acryflavine stained the bacterial body of botn types in the highest concentration, but not in a low concentration.

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The nature of red type colonies on various selective media and their bilogical proper ties on the agar and Endo media were studied in comparison with white type using 13 red type strains out of standard salmonella strains preserved in our hospital.
Though the growth on SS as well as EMB media was possible for both types, the red type growed worse than the white. On the EMB medium no variation of bacterial body was found, while some of the red type strains did not grow at all on the SS medium. Even when they did, both types, particularly the red demonstrated variation of bacterial body. On the BTB medium, the Endo medium improved by Notake, the Kligler medium and the saccharose-mannitol medium they grew well, showing no difference between both types.
There existed almost no difference between them in the biological tests such as fer mentation of urea, indol reaction, methyl red test, Voges-proskauer test, sodium citrate test, milk coagulation test, gelatin liquefaction test and fermentation of carbohydrate.
The motility of the bacilli cultivated in the broth culture was active in the hanging drop, no difference being observed between both types. The red type cultivated on the Endo medium was found very inactive in the hanging drop, whereas the white type demonstrated no fall of motility. Both types, when cultivated on the agar medium, showed no definite difference to each other concerning the catalase and dehydrogenase activity against glucose, mannitol, glycerol, sodium citrate, sodium acetate and sodium succinate.
These activities of the red type, when cultivated on the Endo medium were considerably lowered, while those of the white type were not.
The reduction of those fermentative activities of the red type strains seems to have been caused by lowered vitality, because fuchsin did not impede the dehydrogenase activity of these strains.

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