Effect of Glucuronic Acid and of Sodium Ethylmercurithio-Salicylate on Eruption-Producing Activity and Infectivity of Vaccinia Virus
1959 Volume 33 Issue 8 Pages 752-757
Details
1959 Volume 33 Issue 8 Pages 752-757
The virus treated with varying doses of glucuronolactone or sodium ethylmercurithiosalicylate (Merzonin: Takeda Co.) at 37°C for 4 hours was inoculated intradermally into rabbit and into chick chorioallantoic membrane. The lesions in rabbit were measured every day for a week and pocks of chorioallantoic membrane were counted after 72 hours' incubation. The results are summarized as follows:
1. More virus was required for producing skin lesion in rabbit than for producing pocks on chorioallantoic membrane.
2. The skin-lesion-producing activity was closely related to the infectivity of the virus.
3. The inactivation of the virus by glucuronolactone may be due to the effect of the hydrogen ions of the solution.
4. Chlorpromazine or tetraethylammonium bromide, administered previously to the rabbit, had no effect on the skin-lesion-producing activity of the virus.
5. These results conflict with the data of McCrea and Duran-Reynals (Science, 118: 93, 1953).
