Studies on the Effects of Hypophyseal and Adrenocortical Hormones on Enzymes of the TCA Cycle in the Brain Tissue of Japanese B Encephalitis Mice
1960 Volume 34 Issue 8 Pages 855-871
Details
1960 Volume 34 Issue 8 Pages 855-871
In order to study the effects of ACTH-Z and Decadron on enzymes of the TCA cycle of the Mice inoculated with japanese B encephalitis virus, the Mb reduction activity and the Qo2 value were measured by means of the Thunberg method and Warburg apparatus using the homogenates of brain tissue of these mice added with lactic, pyruvic, citric and succinic acids.
The results were as follows:
1) The Mb reduction activities of brain tissue of healthy mice for lactic, pyruvic, citric and succinic acids were 5.58, 6.57, 3.52 and 13.40, respectively ; the Q02 values were 3.28, 3.96, 2.01 and 4.42, respectively.
2) ACTH-Z and Decadron demonstrated a tendency of increasing the Mb reduction activities of healthy mice for all the substrates mentioned above and induced a marked increase of the Qo2 values.
3) In brain tissue of the japanese B encephalitis mice, the substrate oxidating ability was strongly decreased day by day in the Mb reduction as well as in the Qo2value against lactic and pyruvic acids and in the Qo2 value against citric acid. The succinic acid oxidating ability, however, was well maintained in the Mb reduction as well as in the Qo2 value. Consequently, a marked impairment of metabolism in the initial stage of the TCA cycle was observed in the brain tissue of japanese Bencephalitis mice.
4) By the use of ACTH-Z and Decadron, the decrease of Mb reduction ability as well as Qo2 value was markedly inhibited in the brain tissue of japanese B encephalitis mice.
It was concluded that these hormones seemed to have beneficial effects on the tissue respiration for a relatively long time, until almost the end of the disease.
